Chinese General Practice ›› 2024, Vol. 27 ›› Issue (18): 2225-2232.DOI: 10.12114/j.issn.1007-9572.2023.0386
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Received:
2023-04-28
Revised:
2023-08-14
Published:
2024-06-20
Online:
2024-03-22
Contact:
ZHANG Junmei
通讯作者:
张军梅
作者简介:
作者贡献:
李萧纹提出主要研究目标,负责研究的构思与设计,研究的实施,撰写论文;陈文文、黄明坤进行数据收集与整理,统计学处理,图、表的绘制与展示;闫福华、莫朝伦进行论文的修订;李萧纹、张军梅负责最终版本修订,对论文负责。
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URL: https://www.chinagp.net/EN/10.12114/j.issn.1007-9572.2023.0386
基因 | 引物 |
---|---|
ZO-1 | F:5'-GCCGCTAAGAGCACAGCAA-3' |
R:5'-TCCCCACTCTGAAAATGAGGA-3' | |
闭合蛋白 | F:5'-TTGAAAGTCCACCTCCTTACAGA-3' |
R:5'-CCGGATAAAAAGAGTACGCTGG-3' | |
IL-17A | F:5'-GAAGGCCCTCAGACTACCTCAA-3' |
R:5'-TCATGTGGTGGTCCAGCTTTC-3' | |
IL-10 | F:5'-TGCTATGCTGCCTGCTCTTA-3' |
R:5'-TCATTTCCGATAAGGCTTGG-3' | |
TNF-α | F:5'-AGGGTCTGGGCCATAGAACT-3' |
R:5'-CCACCACGCTCTTCTGTCTAC-3' | |
TLR4 | F:5'-AGAAATTCCTGCAGTGGGTCA-3' |
R:5'- TCTCTACAGGTGTTGCACATGTCA-3' | |
GAPDH | F:5'-AGGTCGGTGTGAACGGATTTG-3' |
R:5'-TGTAGACCATGTAGTTGAGGTCA-3' |
Table 1 qPCR primer sequence
基因 | 引物 |
---|---|
ZO-1 | F:5'-GCCGCTAAGAGCACAGCAA-3' |
R:5'-TCCCCACTCTGAAAATGAGGA-3' | |
闭合蛋白 | F:5'-TTGAAAGTCCACCTCCTTACAGA-3' |
R:5'-CCGGATAAAAAGAGTACGCTGG-3' | |
IL-17A | F:5'-GAAGGCCCTCAGACTACCTCAA-3' |
R:5'-TCATGTGGTGGTCCAGCTTTC-3' | |
IL-10 | F:5'-TGCTATGCTGCCTGCTCTTA-3' |
R:5'-TCATTTCCGATAAGGCTTGG-3' | |
TNF-α | F:5'-AGGGTCTGGGCCATAGAACT-3' |
R:5'-CCACCACGCTCTTCTGTCTAC-3' | |
TLR4 | F:5'-AGAAATTCCTGCAGTGGGTCA-3' |
R:5'- TCTCTACAGGTGTTGCACATGTCA-3' | |
GAPDH | F:5'-AGGTCGGTGTGAACGGATTTG-3' |
R:5'-TGTAGACCATGTAGTTGAGGTCA-3' |
组别 | 只数 | 第1周 | 第2周 | 第3周 | 第4周 | 第5周 | 第6周 |
---|---|---|---|---|---|---|---|
N组 | 8 | 21.05±0.83 | 21.44±1.05 | 21.11±1.29 | 20.36±0.94 | 22.89±1.85 | 23.79±1.72 |
DM组 | 8 | 21.05±0.83 | 23.55±0.89a | 25.85±2.14a | 28.74±2.02a | 27.48±1.22a | 25.69±1.35a |
Pg.组 | 8 | 21.00±0.64 | 21.50±0.85b | 20.10±0.55b | 22.08±1.59b | 22.50±2.52b | 23.88±1.86b |
PD组 | 8 | 20.88±0.72 | 23.03±1.53a | 25.38±1.05ac | 29.26±2.40ac | 28.04±1.92ac | 25.45±0.94ac |
F值 | 0.012 | 7.403 | 35.835 | 50.012 | 18.471 | 3.563 | |
P值 | 0.998 | <0.01 | <0.01 | <0.01 | <0.01 | 0.027 |
Table 2 Changes of body mass in mice before irrigation
组别 | 只数 | 第1周 | 第2周 | 第3周 | 第4周 | 第5周 | 第6周 |
---|---|---|---|---|---|---|---|
N组 | 8 | 21.05±0.83 | 21.44±1.05 | 21.11±1.29 | 20.36±0.94 | 22.89±1.85 | 23.79±1.72 |
DM组 | 8 | 21.05±0.83 | 23.55±0.89a | 25.85±2.14a | 28.74±2.02a | 27.48±1.22a | 25.69±1.35a |
Pg.组 | 8 | 21.00±0.64 | 21.50±0.85b | 20.10±0.55b | 22.08±1.59b | 22.50±2.52b | 23.88±1.86b |
PD组 | 8 | 20.88±0.72 | 23.03±1.53a | 25.38±1.05ac | 29.26±2.40ac | 28.04±1.92ac | 25.45±0.94ac |
F值 | 0.012 | 7.403 | 35.835 | 50.012 | 18.471 | 3.563 | |
P值 | 0.998 | <0.01 | <0.01 | <0.01 | <0.01 | 0.027 |
组别 | 只数 | 第7周 | 第8周 | 第9周 | 第10周 | 第11周 |
---|---|---|---|---|---|---|
N组 | 8 | 25.55±1.14 | 25.91±0.98 | 26.26±0.88 | 26.08±0.78 | 26.08±0.83 |
DM组 | 8 | 25.84±1.66 | 24.39±1.98 | 23.46±2.25a | 22.20±1.51a | 20.91±1.64a |
Pg.组 | 8 | 25.39±0.98 | 26.15±0.74 | 26.06±0.79b | 25.23±1.37b | 24.33±0.91b |
PD组 | 8 | 25.60±1.56 | 24.75±1.04 | 22.16±1.59ac | 20.68±1.22ac | 18.96±1.44abc |
F值 | F交互=32.544,F组间=23.272,F时间=42.092 | |||||
P值 | P交互<0.01,P组间<0.01,P时间<0.01 |
Table 3 Changes of body mass in mice after irrigation
组别 | 只数 | 第7周 | 第8周 | 第9周 | 第10周 | 第11周 |
---|---|---|---|---|---|---|
N组 | 8 | 25.55±1.14 | 25.91±0.98 | 26.26±0.88 | 26.08±0.78 | 26.08±0.83 |
DM组 | 8 | 25.84±1.66 | 24.39±1.98 | 23.46±2.25a | 22.20±1.51a | 20.91±1.64a |
Pg.组 | 8 | 25.39±0.98 | 26.15±0.74 | 26.06±0.79b | 25.23±1.37b | 24.33±0.91b |
PD组 | 8 | 25.60±1.56 | 24.75±1.04 | 22.16±1.59ac | 20.68±1.22ac | 18.96±1.44abc |
F值 | F交互=32.544,F组间=23.272,F时间=42.092 | |||||
P值 | P交互<0.01,P组间<0.01,P时间<0.01 |
组别 | 只数 | 第1周 | 第2周 | 第3周 | 第4周 | 第5周 | 第6周 |
---|---|---|---|---|---|---|---|
N组 | 8 | 5.91±0.46 | 6.09±0.38 | 6.41±0.56 | 6.09±0.68 | 6.26±0.53 | 5.83±0.66 |
DM组 | 8 | 6.08±0.68 | 6.43±0.47 | 6.64±0.47 | 7.51±0.18a | 13.34±1.20a | 14.26±1.12a |
Pg.组 | 8 | 6.06±0.66 | 6.06±0.40 | 6.46±0.60 | 5.86±0.60b | 6.28±0.48b | 5.96±0.78b |
PD组 | 8 | 5.96±0.95 | 6.28±0.66 | 7.13±0.31ac | 7.19±0.84ac | 13.75±1.14ac | 14.19±1.59ac |
F值 | 0.098 | 0.972 | 3.390 | 13.480 | 173.660 | 153.534 | |
P值 | 0.960 | 0.420 | 0.032 | <0.01 | <0.01 | <0.01 |
Table 4 Changes of fasting plasma glucose in mice before irrigation
组别 | 只数 | 第1周 | 第2周 | 第3周 | 第4周 | 第5周 | 第6周 |
---|---|---|---|---|---|---|---|
N组 | 8 | 5.91±0.46 | 6.09±0.38 | 6.41±0.56 | 6.09±0.68 | 6.26±0.53 | 5.83±0.66 |
DM组 | 8 | 6.08±0.68 | 6.43±0.47 | 6.64±0.47 | 7.51±0.18a | 13.34±1.20a | 14.26±1.12a |
Pg.组 | 8 | 6.06±0.66 | 6.06±0.40 | 6.46±0.60 | 5.86±0.60b | 6.28±0.48b | 5.96±0.78b |
PD组 | 8 | 5.96±0.95 | 6.28±0.66 | 7.13±0.31ac | 7.19±0.84ac | 13.75±1.14ac | 14.19±1.59ac |
F值 | 0.098 | 0.972 | 3.390 | 13.480 | 173.660 | 153.534 | |
P值 | 0.960 | 0.420 | 0.032 | <0.01 | <0.01 | <0.01 |
组别 | 只数 | 第7周 | 第8周 | 第9周 | 第10周 | 第11周 |
---|---|---|---|---|---|---|
N组 | 8 | 5.61±0.64 | 5.98±0.69 | 6.15±0.46 | 6.46±0.58 | 6.08±0.63 |
DM组 | 8 | 13.91±2.56a | 14.30±1.71a | 14.46±2.04a | 13.84±1.62a | 14.26±1.32a |
Pg.组 | 8 | 6.45±0.46b | 6.58±0.58b | 6.95±0.53b | 7.45±1.23b | 8.15±1.90b |
PD组 | 8 | 13.73±1.04ac | 14.05±0.57ac | 14.23±1.30ac | 15.84±1.77abc | 17.14±2.20abc |
F值 | F交互=2.187,F组间=358.936,F时间=6.371 | |||||
P值 | P交互=0.021,P组间<0.01,P时间<0.01 |
Table 5 Changes of fasting plasma glucose in mice after irrigation
组别 | 只数 | 第7周 | 第8周 | 第9周 | 第10周 | 第11周 |
---|---|---|---|---|---|---|
N组 | 8 | 5.61±0.64 | 5.98±0.69 | 6.15±0.46 | 6.46±0.58 | 6.08±0.63 |
DM组 | 8 | 13.91±2.56a | 14.30±1.71a | 14.46±2.04a | 13.84±1.62a | 14.26±1.32a |
Pg.组 | 8 | 6.45±0.46b | 6.58±0.58b | 6.95±0.53b | 7.45±1.23b | 8.15±1.90b |
PD组 | 8 | 13.73±1.04ac | 14.05±0.57ac | 14.23±1.30ac | 15.84±1.77abc | 17.14±2.20abc |
F值 | F交互=2.187,F组间=358.936,F时间=6.371 | |||||
P值 | P交互=0.021,P组间<0.01,P时间<0.01 |
组别 | 只数 | 0 min | 30 min | 60 min | 90 min | 120 min |
---|---|---|---|---|---|---|
N组 | 8 | 6.01±0.68 | 12.31±2.01 | 10.63±1.73 | 7.70±0.74 | 6.16±0.55 |
DM组 | 8 | 12.43±1.55a | 20.00±4.89a | 18.35±4.30a | 15.54±2.26a | 13.95±1.32a |
Pg.组 | 8 | 7.56±0.93b | 15.09±1.55b | 13.46±1.42b | 10.59±1.60b | 9.30±1.55ab |
PD组 | 8 | 15.08±1.29abc | 25.79±3.43abc | 24.01±4.39abc | 20.88±4.74abc | 16.91±1.89abc |
F值 | F交互=3.902,F组间=52.580,F时间=63.235 | |||||
P值 | P交互=0.013,P组间<0.01,P时间<0.01 |
Table 6 Comparison of blood glucose at each time point of OGTT test in 4 groups of mice
组别 | 只数 | 0 min | 30 min | 60 min | 90 min | 120 min |
---|---|---|---|---|---|---|
N组 | 8 | 6.01±0.68 | 12.31±2.01 | 10.63±1.73 | 7.70±0.74 | 6.16±0.55 |
DM组 | 8 | 12.43±1.55a | 20.00±4.89a | 18.35±4.30a | 15.54±2.26a | 13.95±1.32a |
Pg.组 | 8 | 7.56±0.93b | 15.09±1.55b | 13.46±1.42b | 10.59±1.60b | 9.30±1.55ab |
PD组 | 8 | 15.08±1.29abc | 25.79±3.43abc | 24.01±4.39abc | 20.88±4.74abc | 16.91±1.89abc |
F值 | F交互=3.902,F组间=52.580,F时间=63.235 | |||||
P值 | P交互=0.013,P组间<0.01,P时间<0.01 |
组别 | 只数 | OGTT AUC(mmol/min) | LPS[M(P25,P75),EU/mL] |
---|---|---|---|
N组 | 8 | 1 102±96 | 1.55(1.46,3.17) |
DM组 | 8 | 2 012±302a | 3.52(2.95,4.50) |
Pg.组 | 8 | 1 427±137b | 3.92(3.02,5.49) |
PD组 | 8 | 2 597±394abc | 9.42(5.63,14.10)ab |
F(H)值 | 25.348 | 20.418d | |
P值 | <0.01 | <0.01 |
Table 7 Comparison of AUC of OGTT and serum LPS level among four groups of mice
组别 | 只数 | OGTT AUC(mmol/min) | LPS[M(P25,P75),EU/mL] |
---|---|---|---|
N组 | 8 | 1 102±96 | 1.55(1.46,3.17) |
DM组 | 8 | 2 012±302a | 3.52(2.95,4.50) |
Pg.组 | 8 | 1 427±137b | 3.92(3.02,5.49) |
PD组 | 8 | 2 597±394abc | 9.42(5.63,14.10)ab |
F(H)值 | 25.348 | 20.418d | |
P值 | <0.01 | <0.01 |
组别 | 只数 | ZO-1[M(P25,P75)] | 闭合蛋白 | IL-17A[M(P25,P75)] | IL-10 | TNF-α | TLR4 |
---|---|---|---|---|---|---|---|
N组 | 8 | 1.05(0.70,1.16) | 1.02±0.24 | 1.03(0.40,1.76) | 1.04±0.30 | 1.07±0.39 | 1.06±0.36 |
DM组 | 8 | 0.95(0.40,1.17) | 0.60±0.14a | 0.34(0.15,1.54) | 0.55±0.09a | 1.11±0.38 | 1.67±0.49 |
Pg.组 | 8 | 0.52(0.30,1.23) | 0.74±0.28 | 0.94(0.53,1.38) | 0.58±0.19a | 1.14±0.76 | 2.46±1.09a |
PD组 | 8 | 0.36(0.26,0.46)a | 0.20±0.05abc | 0.16(0.12,0.34)ac | 0.60±0.13a | 2.97±1.10abc | 3.94±1.95a |
F(H)值 | 9.940d | 23.867 | 9.571d | 11.010 | 13.459 | 9.249 | |
P值 | 0.019 | <0.01 | 0.023 | <0.01 | <0.01 | <0.01 |
Table 8 Comparison of mRNA expression levels of colonic tight junction protein and inflammatory factors among 4 groups of mice
组别 | 只数 | ZO-1[M(P25,P75)] | 闭合蛋白 | IL-17A[M(P25,P75)] | IL-10 | TNF-α | TLR4 |
---|---|---|---|---|---|---|---|
N组 | 8 | 1.05(0.70,1.16) | 1.02±0.24 | 1.03(0.40,1.76) | 1.04±0.30 | 1.07±0.39 | 1.06±0.36 |
DM组 | 8 | 0.95(0.40,1.17) | 0.60±0.14a | 0.34(0.15,1.54) | 0.55±0.09a | 1.11±0.38 | 1.67±0.49 |
Pg.组 | 8 | 0.52(0.30,1.23) | 0.74±0.28 | 0.94(0.53,1.38) | 0.58±0.19a | 1.14±0.76 | 2.46±1.09a |
PD组 | 8 | 0.36(0.26,0.46)a | 0.20±0.05abc | 0.16(0.12,0.34)ac | 0.60±0.13a | 2.97±1.10abc | 3.94±1.95a |
F(H)值 | 9.940d | 23.867 | 9.571d | 11.010 | 13.459 | 9.249 | |
P值 | 0.019 | <0.01 | 0.023 | <0.01 | <0.01 | <0.01 |
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