Correlation between Cervicovaginal Microbiota Variation and Persistent HPV Infection

doi:10.12114/j.issn.1007-9572.2020.00.642

Abstract

Abstract: Background　Early differentiation of persistent and transient HPV infection is important in diagnosis and treatment of cervical cancer.It will provide help for the early intervention of cervical cancer，which has important clinical significance.Changes in cervicovaginal microbiota may induce inflammatory microenvironment and persistent chronic inflammation，or may be associated with persistent HPV infection.Objective　To determine the correlation between cervicovaginal microbiota variation and persistent HPV infection.Methods　16s rDNA sequencing was used to determine the cervicovaginal microbiota variation in the samples of women with persistent HPV infection（n=6），transient HPV infection（n=4） and HPV negative（n=5） selected from those undergoing gynecological examination or treatment in Qingdao Central Hospital during January to June 2018.Alpha diversity was applied in analyzing complexity of species diversity.T-test and LEfSe analysis were used to determine the variation of cervicovaginal microbiota among different groups.Results　The structure of cervicovaginal microbiota of healthy and transiently infected individuals was relatively single，Firmicutes occupied the main composition.However，that of those with persistent HPV infection presented a complicated trend and the abundance of Proteobacteria and Bacteroidetes was higher.Bacteria with significant differences between persistent and transient HPV infection included Acinetobacter，Prevotella，Pseudomonas and Sphingomonas.Conclusion　The reproductive tract flora of patients with persistent HPV infection is complex，and Acinetobacter，Prevotella，Pseudomonas and Sphingomonas are related to persistent HPV infection.

Key words: Cervicovaginal microbiota, Human papilloma virus, Reproductive tract infections, Microbiota variation

摘要： 背景　在宫颈癌的临床诊疗中，早期区分人乳头瘤病毒（HPV）持续感染和一过性感染是重要的问题，该问题的解决将为宫颈癌的早期干预提供帮助，具有重要的临床意义。生殖道菌群改变可诱发炎性微环境和持续的慢性炎症，或与HPV持续感染相关。目的　探讨女性生殖道菌群改变与HPV持续感染的相关性。方法　选取2018年1—6月于青岛市中心医院妇科体检或诊治的女性15例，根据HPV感染情况共分为HPV持续感染组6例、HPV一过性感染组4例、HPV阴性组5例。收集其生殖道样本，进行16s rDNA扩增子测序后，基于有效数据进行聚类分析和物种分类分析。根据聚类分析结果，进行物种丰度、Alpha多样性计算分析，探究不同样本或组别间菌群结构差异；随后采用t检验、LEfSe对不同分组样本的菌种组成和菌群结构进行差异性检验。结果　HPV阴性组和HPV一过性感染组的生殖道菌群结构相对单一，厚壁菌门占据主要地位；而HPV持续感染组的生殖道菌群结构呈现复杂化趋势，变形菌门和拟杆菌门的丰度较高。HPV持续感染组和HPV一过性感染组具有显著性差异的菌属包括不动杆菌属、普雷沃菌属、假单胞菌属和鞘氨醇单胞菌属等。结论　HPV持续感染患者生殖道菌群结构复杂，且不动杆菌属、普雷沃菌属、假单胞菌属和鞘氨醇单胞菌属等与HPV持续感染相关。

关键词: 生殖道菌群, 人乳头瘤病毒, 生殖道感染, 差异菌种

BI Qingqing,ZHU Jie,ZHANG Lei, et al. Correlation between Cervicovaginal Microbiota Variation and Persistent HPV Infection　[J]. Chinese General Practice, 2021, 24(9): 1102-1106. DOI: 10.12114/j.issn.1007-9572.2020.00.642.
毕庆庆,朱婕,张磊等. 女性生殖道菌群改变与人乳头瘤病毒持续感染的相关性研究[J]. 中国全科医学, 2021, 24(9): 1102-1106. DOI: 10.12114/j.issn.1007-9572.2020.00.642.

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