关键词: 疲劳, 磁珠, 生物标志物, 唾液, 肽图谱

Abstract: Objective　In order to provide a reference basis for exploring convenient and stable fatigue markers，we studied various high-molecular-weight peptide fragments under the fatigue state and non-fatigue state using in the saliva using time of flight mass spectrometry.Methods　The saliva samples were collected from 10 healthy volunteers and the flight time of mass spectrometry was conducted using two kinds of sample treatment methods，the magnetic beads enrichment（MB） and direct detection of stock solution.This was followed by modeling via the mass spectrums of MB and supernatant（stock solution） directly collected after centrifugation.Results　Both MB and direct sampling produced good spectrograms between 2 000 and 150 00 Da，while some peaks were lost in the enrichment.The early and late spectrograms were different in each individual.Due to the limited sample size，the cross validation was not conducted，however two spectrograms were taken for each sample，and 20 early and 20 late spectrograms were used for modeling analysis.Three differential peptides was found in the two groups of stock solution samples.The cross validity of MB model was 92.06%，while that of stock solution model was 95.49%.Conclusion　There were differential peaks within the molecular weight of 2 000-15 000 Da，in different degrees of fatigue which provided a scientific basis for further realization of the convenient fatigue detection method based on biosensor technique，with important theoretical and practical significance.

Key words: Fatigue, Magnetic bead, Biomarkers, Saliva, Peptide mapping