关键词: 血管紧张素原, 肾小球滤过率, 肾功能衰竭, 慢性, 炎性细胞浸润

Abstract: Objective　To analyse the expression of urinary angiotensinogen（uAGT） and its relationship with renal interstitial inflammatory cell infiltration in patients with kidney failure.Methods　The clinical and renal histopathologic data in 77 patients with kidney failure admitted to the Department of Nephrology，the First Affiliated Hospital of Zhengzhou University were collected between December 2014 to April 2016.Twelve healthy people admitted to this hospital were selected as the control group.The clinical data of the renal failure group and the control group were collected，and the pathological data of the renal failure group were collected.Katafuchi score standard was used for semi quantitative score of glomerular sclerosis，interstitial inflammatory cell infiltration，interstitial fibrosis and tubular atrophy，and the expression level of uAGT was assessed by Enzyme-linked Immunosorbent Assay（ELISA）．The correlation between uAGT and clinical and pathological data was analysed.The receiver operating characteristic （ROC） curve of uAGT for the diagnosis of renal pathological damage was drawn and the area under the ROC curve （AUC） was calculated.Results　The levels of hemoglobin（Hb），serum albumin （Alb），estimated glomerular filtration rate （eGFR），and high density lipoprotein（HDL） in kidney failure group were lower than those in the control group，while levels of urea，uric acid（UA），creatinine（Cr），total cholesterol （TC），triacylglycerol （TG），uAGT in kidney failure group were higher than those in the control group（P<0.05）．There was a negative correlation between uAGT and Hb，Alb，urea，eGFR（rs=-0.427，-0.379，-0.266，-0.254，all P<0.05），and a positive correlation between uAGT and urinary protein in patients with kidney failure（rs=0.445，P<0.001）.There was a　postive correlation between uAGT and glomerular sclerosis（rs=0.270，P=0.018），interstitial inflammatory cell infiltration（rs=0.401，P<0.001），interstitial fibrosis（rs=0.273，P=0.016）and tubular atrophy（rs=0.272，P=0.017），respectively.For diagnosing glomerular sclerosis，greater accuracy，interstitial fibrosis ，and tubular atrophy，the AUC of the uAGT level was 0.627，0.689，0.627，0.639．Conclusions　UAGT increased significantly in patients with renal failure，and the uAGT showed a positive correlation with interstitial inflammatory cell infiltration and can reflected its severity.uAGT level was able to identify interstitial inflammatory cell infiltration with greater accuracy，and may be used as a non-invasive biomarker of interstitial inflammatory cell infiltration in patients with kidney failure.

Key words: Angiotensinogen；Glomerular filtration rate；Kidney failure, chronic disease；Inflammatory cell infiltration