关键词: 肺肿瘤, 小鼠, 环磷酰胺, 免疫

Abstract: Objective　To explore the role of yifeizhuji decoction（YFZJD） combined with cyclophosphamide（CTX） in the treatment of lung carcinoma and immune regulation based on observing its effects on the tumor volume，thymus indices and percentage of lymphocytes in the Lewis lung cancer mouse model.Methods　We conducted this study from April to May 2016.55 male C57BL/6 mice were selected，5 of them were inoculated with Lewis lung cancer cells to prepare tumor cell suspension，other 50 were randomly divided into model group（40） and normal control group（10）．Model group mice received subcutaneous inoculation of Lewis tumor cell suspension in the right forelimb armpit to establish the lung cancer mice model.When the models were successfully developed，mice from model group were divided into YFZJD subgroup（10），CTX subgroup（10），YFZJD combined with CTX subgroup（10），model control subgroup（10） by means of random number table.From the second day after modeling，YFZJD subgroup and YFZJD combined with CTX subgroup received a 14-day sequential therapy of intragastric administration of YFZJD（14.95 g/kg per day），and the model control subgroup and the normal control group received a 14-day sequential therapy of intragastric administration of 0.9% sodium chloride solution（with the volume equal to that of YFZJD per day）．The CTX subgroup only received and YFZJD combined with CTX subgroup additionally received intraperitoneal injection of CTX（40 mg/kg） at 72 h and on the 10th day after inoculation.At the end of intervention，the tumor diameter was measured and the tumor volume was calculated；the body weight and thymus weight were measured，the thymus indices were calculated；the percentages of CD4+，CD8+ T-lymphocytes and B cells were detected by flow cytometry.Results　Smaller tumor was found in YFZJD subgroup and YFZJD combined with CTX subgroup instead of the model control subgroup（P<0.05），and in the YFZJD combined with CTX subgroup instead of CTX subgroup（P<0.05）．The normal control group had greater body weight compared with other 4 subgroups（P<0.05）．The YFZJD subgroup had greater thymus weight and higher levels of thymus indices than the normal control group as well as the CTX subgroup（P<0.05）．The percentages of CD4+，CD8+ T-lymphocytes in the model control subgroup were less than those of the normal control group as well as those of other 3 subgroups（P<0.05）．The YFZJD combined with CTX subgroup owned higher percentages of CD4+，CD8+ T-lymphocytes compared with the CTX subgroup（P<0.05）．The model control subgroup demonstrated a higher percentage of B cells compared with the normal control group and other 3 subgroups（P<0.05）．The percentage of B cells in YFZJD subgroup was higher than that of the CTX subgroup（P<0.05）．Conclusion　The combined use of YFZJD with CTX can effectively inhibit the growth of Lewis lung cancer tumor and improve the CTX-induced immunosuppression by upregulating the thymus indices as well as the percentages of CD4+ and CD8+ T-lymphocytes and B cells.

Key words: Lung neoplasms, Mice, Cyclophosphamide, Immunity