关键词: 癌, 非小细胞肺, 受体, 雌激素, 微RNAs, 早期诊断

Abstract: Objective　To investigate the change and correlation of serum estrogen and miR-365 levels in non-small cell lung cancer（NSCLC） patients，and to provide new insight for the early diagnosis and treatment monitoring of this disorder.Methods　Fifty-seven patients who underwent resection of primary NSCLC in the Affiliated Renhe Hospital of China Three Gorges University from March 2015 to April 2016 were selected as the NSCLC group；50 healthy volunteers who received physical examinations in the hospital during the same period were selected as the control group.General information of the participants was collected，and the expression levels of serum estrogen and miR-365 were determined.The correlation between serum estrogen levels and miR-365 expression levels were then analyzed.Results　The serum estrogen level in the NSCLC group was significantly higher than that in the control group（t=2.73，P=0.01），while miR-365 expression level in the NSCLC group was significantly lower than that in the control group（t=2.65，P=0.02）．In the NSCLC group，patients with squamous cell carcinoma and adenosquamous carcinoma had higher expression of miR-365 than patients with adenocarcinoma（P<0.05），patients with poor differentiation was associated with a lower expression level of miR-365 than patients with high differentiation（P<0.05）．In the NSCLC group，for the TNM stage，patients in stages Ⅰ-Ⅱ had a higher expression level of miR-365 than patients in stages Ⅲ-Ⅳ（P<0.05）．In the NSCLC group，the expression level of miR-365 in patients with lymph node metastasis was lower than those without lymph node metastasis（P<0.05）．Moreover，in the NSCLC group，the serum estrogen level showed a negative correlation with the miR-365 expression level（r=-0.574，P=0.02）．Conclusion　Serum estrogen levels increased and miR-365 expression levels decreased in patients with NSCLC.The serum estrogen level was negatively correlated with the miR-365 expression level.Both serum estrogen and miR-365 levels were important biomarkers for the early diagnoses of NSCLC and the prediction of patient outcomes.

Key words: Carcinoma, non-small-cell lung cancer；Receptors, estrogen；MicroRNAs；Early diagnosis